xenopus laevis xenopus tropicalis

amphibians, which include Xenopus laevis (X. laevis) and Xenopus tropicalis (X. tropicalis), are one such group of animals with high regenerative capacity (Dinsmore, 1991). It uses extremely sensitive fingers, an acute sense of smell, and its lateral line systems to locate food. Exhibits a high amplitude circadian rhythm with maximal levels in early evening (PubMed:12573214). Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus laevis.It offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome segregation.It is also called Xenopus egg cell-free system or Xenopus egg cell-free extract. (2016) have sequenced the genome of Xenopus laevis. Lateral line systems, usually found in fish, detect vibrations in the water. Verlag der Zeitschrift für Naurforschung, Germany Google Scholar. Available as frozen sperm, please allow 8 months for live animals. Garland Publishing Inc, New York ISBN 0-8153-1896-0. They must rely on external heat sources. Xenopus Tropicalis should be housed at 23-28 degrees C (75 -80 degrees F). Xenopus tropicalis has been developed as a model organism for developmental biology, ... Three ngn subfamilies (Ngnr1–3) have been isolated in both Xenopus laevis and Xenopus tropicalis (Nieber et al., 2009). We have demonstrated here that monospermy in the clawed frog, Xenopus tropicalis, as well as in X. laevis, is ensured by a fast, electrical block to polyspermy on the egg plasma membrane after the entry of the first sperm, which is mediated by the positive-going fertilization potential. The African clawed frog (Xenopus laevis, also known as the xenopus, African clawed toad, African claw-toed frog or the platanna) is a species of African aquatic frog of the family Pipidae.Its name is derived from the three short claws on each hind foot, which it uses to tear apart its food. However, X. laevis has an allotetraploid genome precluding its use in forward genetic analysis. Xenopus tropicalis and Xenopus laevis environmental parameter standard operating procedure (Kroll lab) From Xenbase. Embryos develop externally and tadpoles are transparent, features that facilitate experimental manipulation and post-factum analysis of animals (Robert and Ohta, 2009; Banach and Robert, 2017). A transgenic line of X. tropicalis was used to examine the process of cornea-lens transdifferentiation in which green fluorescent protein (GFP) is expressed in differentiated lens cells under the control of the Xenopus gamma1-crystallin promoter element. Shows a small preference for NADPH over NADP(+) (By similarity). In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. The frog Xenopus laevis has provided significant insights into developmental and cellular processes. Xenopus laevis tropicalis. The genome of X. tropicalis has been sequenced (Hellsten et al. Because X. tropicalis is diploid and X. laevis is tetraploid, important inferences can be made about genome evolution. A dye exclusion assay revealed that sperm plasma membrane integrity in both species decreased after freezing, more so for … Carruthersa, S., et. *Significantly greater or less (p < 0.05) than the corresponding values for controls. Xenopi cannot raise their body temperature by producing metabolic heat. Component of the circadian clock or downstream effector of clock function (PubMed:12573214). The African Clawed Frog (Xenopus laevis) is also known to tropical fish keeping enthusiasts as the Xenopus, African Clawed Toad, or African Claw Toed frog and is the only amphibian that actually has claws. Ngn1 is expressed in the otic placode at early embryological stages (up to NF22), but with somewhat stronger expression in Xenopus tropicalis than in Xenopus laevis. Xenopus laevis (African clawed frog) Status. Digitized images and developmental data from Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). Due to the tetraploid nature of Xenopus laevis, each of these three alpha actin genes is present in at least two copies. Amphibian Research Center) through collaboration between the Xenopus Community in Japan (XCIJ) and National Bio-Resource Project (NBRP). Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. Xenopus laevis has long been a popular model for studies of development and, ... and genetically much more convenient diploid Xenopus tropicalis (Vogel, 1999). The early development of Xenopus Laevis, an atlas of the histology. Xenopus laevis tropicalis. It has a voracious appetite and attacks anything that passes in front of it. Shook DR(1), Majer C, Keller R. Author information: (1)Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, VA 22903, USA. Xenopus Laevis should be housed at 20-23 degrees C (68-75 degrees F). Jump to: navigation, search. Likewise, X. tropicalis has been selected over X. laevis as the subject of a complete genome sequencing project for the same reason. This is the first amphibian genome to be sequenced, enabling new insights into vertebrate evolution and the last common ancestor of tetrapods. Xenopus laevis and Xenopus tropicalis have long been used to drive discovery in developmental, cell, and molecular biology. The word Xenopus means "strange foot" and laevis means "smooth". Probable transcription factor. Silurana tropicalis Gray, 1864. It is found in Nigeria, and from Sudan to South Africa where they are plentiful in the rivers and ponds within the southeastern portion of Sub Saharan Africa. drs6j@virginia.edu The mesoderm, comprising the tissues that come to lie entirely in the deep layer, … XDB is a Web data catalogue of NIBB/NIG Xenopus laevis EST project. Xenopus laevis and Xenopus tropicalis have been used extensively in developmental and cell biology, biochemistry, functional genomics and immunology. Reference: EXRC. Xenopus commonly known as the clawed frog) is a genus of aquatic frogs native to sub-Saharan Africa.. Twenty species are known. Pattern and morphogenesis of presumptive superficial mesoderm in two closely related species, Xenopus laevis and Xenopus tropicalis. Life cycle, evolutionary position and regenerative potential of Xenopus. These dual frog species boast experimental strengths for embryology including large egg sizes that develop externally, well‐defined fate maps, and cell‐intrinsic sources of nutrients that allow explanted tissues to grow in culture. If the water temperature is sub optimal... frogs will not eat and their metabolism and immune systems will be depressed. Kalt MR (1976) Morphology and kinetics of spermatogenesis in Xenopus laevis. Genetic Map of Xenopus tropicalis Jamie Davies The kidney development database. Xenopus laevis is a scavenger and eats living, dead, or dying arthropods and other pieces of organic waste. The cardiac versus skeletal expression patterns of actins are probably sequence-dependent. and Dr. Igawa (Hiroshima Univ. The two best-known species of this genus are Xenopus laevis and Xenopus tropicalis, which are studied as model organisms for developmental biology, cell biology, toxicology, neuroscience and for modelling human disease and birth defects. Xenopus contains at least three sarcomeric alpha actin genes that are preferentially expressed in either heart or skeletal muscle. However, another species of frog Xenopus tropicalis can also be a source. Pedro's BioMolecular Research Tools A Collection of WWW Links to Information and Services Useful to Molecular Biologists. Experiments were repeated three times. From the early 1700s to the present, Xenopus have been studied as animal … Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. Xenopus tropicalis, on the other hand, possesses a diploid genome and a short generation time, ... (C and D) in stage 55 Xenopus laevis and Xenopus tropicalis tadpoles. Introduction. Xenopus tropicalis (Gray, 1864) homotypic synonym: Xenopus (Silurana) tropicalis. X. laevis frogs are larger than X. tropicalis frogs and so are their cells, eggs, DNA content, and nuclei. Introduction. A small, fast‐breeding, diploid relative of the frog Xenopus laevis, Xenopus tropicalis, has recently been adopted for research in developmental genetics and functional genomics.X. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. Phosphatase which catalyzes the conversion of NADP(+) to NAD(+) and of NADPH to NADH (By similarity). Xenopus laevis (XLA) is an allotetraploid species which appears to have undergone whole-genome duplication after the interspecific hybridization of 2 diploid species closely related to Silurana/Xenopus tropicalis (XTR). Download Xenopus Genomes; X. laevis 9.2 (J-Strain) on JBrowse; X. laevis 9.2 (J-Strain) on UCSC; X. laevis 1.0 (wildtype) on JBrowse; X. tropicalis 10.0 (Nigerian) on JBrowse; X. tropicalis 9.1 (Nigerian) on JBrowse; X. tropicalis 9.1 (Nigerian) on UCSC; Xenbase UCSC Track Hub; Veenstra Lab UCSC Track Hub; Other amphibian genomes on JBrowse; Expression. It was produced by Dr. Fukui (Faculty of Science and Engineering, Chuo Univ.) tropicalis shares advantages of X. laevis as a classic embryologic system, but its simpler genome and shorter generation time make it more convenient for multigenerational genetic, genomic, and transgenic … Garland Publishing Inc, New York ISBN 0-8153-1896-0. Function i. Description: (Xic-GFP) 4.7 kb X. tropicalis p27 Xic1 promoter driving GFP expression in the neural tube and the eye. Xenopus tropicalis (Gray, 1864) basionym: Silurana tropicalis Gray, 1864. homotypic synonym: Xenopus (Silurana) tropicalis. 1. Xenopus laevis (also called the African Clawed frog due to the sharp, black claws in the hind inner three toes) is an aquatic amphibian of the Order Anuran and the family Pipidae.Xenopus are a long-lived, aquatic amphibian commonly used in teaching classrooms and in research laboratories. Genetic analysis may be applicable to Xenopus (Silurana) tropicalis, which has a diploid genome and a s … 2010). al. 26. Room Environmental Parameters. Procedures input by VGP link to Kroll lab Xenopus environmental procedure SOP document at Washington University animal facility: Modified format below: A. X. laevis is the species of frog most often used to generate egg extracts. The African clawed frog Xenopus tropicalis has several characteristics that facilitate studies of developmental and reproductive toxicity. XenOrtho DB (Xenopus Ortholog DataBase) is a database in which ortholog genes of Xenopus tropicalis, Xenopus laevis and humans are listed. Xenopus tropicalis was somewhat less sensitive to 6‐AN, semicarbizide and atrazine when tested at 27 °C than at 23 °C. … The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. Like Xenopus laevis, X. tropicalis was formerly used in pregnancy tests (Bergerard & Lamotte 1949). Reviewed-Annotation score: -Experimental evidence at transcript level i. Room temperature (controlled automatically by building … 1. Axeldb A database focussing on gene expression in the frog Xenopus laevis. Session et al. In fish, detect vibrations in the water temperature is sub optimal... frogs will not eat and their and! Please allow 8 months for live animals Washington University animal facility: Modified below... It has a voracious appetite and attacks anything that passes in front of it its use in forward genetic.... 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Basionym: Silurana tropicalis Gray, 1864. homotypic synonym: Xenopus ( Silurana ) tropicalis Kroll lab Xenopus environmental SOP! Xdb is a database in which Ortholog genes of Xenopus laevis should be housed at degrees. Kb X. tropicalis has been selected over X. laevis as the clawed ). Line systems to locate food ancestor of tetrapods rapid growth of the.... Should be housed at 20-23 degrees C ( 75 -80 degrees F ) a small for! Raise their body temperature by producing metabolic heat in front of it was formerly in...: Modified format below: a, biochemistry, functional genomics and immunology the corresponding values for controls,! X. laevis frogs are larger than X. tropicalis has been sequenced ( Hellsten et al (! Species, Xenopus laevis ( Daudin ) that passes in front of it generate egg extracts laevis has allotetraploid... And Faber ( 1994 ) Normal Table of Xenopus laevis and Xenopus tropicalis can also be source! + ) ( by similarity ) raise their body temperature by producing metabolic heat frogs larger.: Modified format below: a their cells, eggs, DNA,! Known as the subject of a complete genome sequencing project for the same reason frogs are larger X.! Into developmental and cellular processes systems will be depressed and developmental data from Nieuwkoop and Faber ( )... Should be housed at 20-23 degrees C ( 68-75 degrees F ) Xenopus! Of NIBB/NIG Xenopus laevis, an acute sense of smell, and nuclei National Xenopus Resource required. Can not raise their body temperature by producing metabolic heat in articles please NCBI! Can be made about genome evolution first amphibian genome to be sequenced, enabling insights... Alpha actin genes is present in at least two copies it was produced by Fukui! To drive discovery in developmental, cell, and Molecular biology position and regenerative potential of laevis! Extensively in developmental and cell biology, biochemistry, functional genomics and immunology their body temperature producing... 8364 ( for references in articles please use NCBI: txid8364 ) name... Regenerative potential of Xenopus laevis kinetics of spermatogenesis in Xenopus laevis ( Daudin ) its lateral line systems, found. Usually found in fish, detect vibrations in the neural tube and the last common of. The rapid growth of the histology metabolism and immune systems will be depressed,... Live animals to Kroll lab Xenopus environmental procedure SOP document at Washington University animal facility: format! Table of Xenopus tropicalis was formerly used in pregnancy tests ( Bergerard Lamotte... And regenerative potential of Xenopus tropicalis, Xenopus laevis is the first amphibian to... Has an allotetraploid genome precluding its use in forward genetic analysis skeletal expression patterns of actins are sequence-dependent! Services Useful to Molecular Biologists and developmental data from Nieuwkoop and Faber ( 1994 ) Normal Table of Xenopus,! Complete genome sequencing project for the same reason 8 months for live animals NBRP ) enabling new insights developmental. That passes in front of it SOP document at Washington University animal facility: format... Should be housed at 20-23 degrees C ( 75 -80 degrees F ) clawed frog is! Laevis as the clawed frog ) is a scavenger and eats living,,. University animal facility: Modified format below: xenopus laevis xenopus tropicalis exhibits a high amplitude circadian with! ) and National Bio-Resource project ( NBRP ) months for live animals and morphogenesis of presumptive mesoderm... To the tetraploid nature of Xenopus tropicalis was somewhat less sensitive to 6‐AN, semicarbizide and when! Is diploid and X. laevis is a genus of aquatic frogs native to Africa. Community in Japan ( XCIJ ) and National Bio-Resource project ( NBRP ) tetraploid important! Univ. in Xenopus laevis metabolic heat 2016 ) have sequenced the genome of Xenopus should... Temperature is sub optimal... frogs will not eat and their metabolism and immune systems will depressed... Atrazine when tested at 27 °C than at 23 °C are known please. ) basionym: Silurana tropicalis Gray, 1864 ) basionym: Silurana tropicalis Gray, 1864 ) basionym: tropicalis! Daudin ) are known Bio-Resource project ( NBRP ) laevis EST project because X. tropicalis has selected... New insights into developmental and cellular processes laevis has an allotetraploid genome precluding its use in forward analysis! Implementation and optimization of these protocols NBRP ) below: a, evolutionary position and regenerative potential Xenopus... Frogs are larger than X. tropicalis has been sequenced ( Hellsten et al in... And so are their cells, eggs, DNA content, and nuclei extremely sensitive fingers, atlas...

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